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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SDC2 Monoclonal antibody, PBS Only

SDC2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A7B2

Cat no : 67088-1-PBS

Synonyms

Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDC2 Monoclonal antibody (67088-1-Ig)

    WB analysis using 67088-1-Ig (same clone as 67088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67088-1-Ig (SDC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SDC2 Monoclonal antibody (67088-1-Ig)

    IHC staining of human liver cancer using 67088-1-Ig (same clone as 67088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SDC2 Monoclonal antibody (67088-1-Ig)

    IHC staining of human liver cancer using 67088-1-Ig (same clone as 67088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SDC2 Monoclonal antibody (67088-1-Ig)

    IHC staining of human colon cancer using 67088-1-Ig (same clone as 67088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SDC2 Monoclonal antibody (67088-1-Ig)

    IHC staining of human prostate cancer using 67088-1-Ig (same clone as 67088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SDC2 Monoclonal antibody (67088-1-Ig)

    IHC staining of human colon cancer using 67088-1-Ig (same clone as 67088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67088-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67088-1-PBS targets SDC2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SDC2 fusion protein Ag5739 相同性解析による交差性が予測される生物種
Full Name syndecan 2
Calculated molecular weight 22 kDa
Observed molecular weight 19 kDa, 22 and 48 kDa
GenBank accession numberBC049836
Gene symbol SDC2
Gene ID (NCBI) 6383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)