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Syndecan-3 Polyclonal antibody

Syndecan-3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10886-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

SDC3, syndecan 3, SYND3, SDCN, N syndecan

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue, A549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10886-1-AP targets Syndecan-3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syndecan-3 fusion protein Ag1317 相同性解析による交差性が予測される生物種
Full Name syndecan 3
Calculated molecular weight 38 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC013974
Gene Symbol Syndecan-3
Gene ID (NCBI) 9672
RRIDAB_2182999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75056
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Protocols

Product Specific Protocols
WB protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IHC protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IF protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IP protocol for Syndecan-3 antibody 10886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Pathol

Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.

Authors - van Oosterwijk Jolieke G JG
humanIHC

Am J Pathol

No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.

Authors - Reijnders Christianne M A CM
humanWB

J Cell Sci

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Authors - Yu-Ja Huang
humanIHC

BMC Cancer

Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.

Authors - Wu Zheng-Sheng ZS
humanIHC

Medicine (Baltimore)

Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3

Authors - Guozhi Yang
mouseIF

Int J Mol Sci

GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway

Authors - Apostolos Mikroulis
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