Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10886-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10886-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10886-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 10886-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10886-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10886-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 10886-1-AP
IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:1000) with mouse lung tissue lysate 4000ug.
IP experiment of A549 using 10886-1-AP
IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:600) with A549 cells lysate 2800ug.
IHC staining of human colon cancer using 10886-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10886-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-12 using 10886-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Syndecan-3 antibody (10886-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 10886-1-AP
Immunofluorescent analysis of MCF-7 cells, using SDC3 antibody 10886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Syndecan-3 Polyclonal antibody

Syndecan-3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10886-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SDC3, syndecan 3, SYND3, SDCN, N syndecan

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue
Positive IP detected in	mouse lung tissue, A549 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-12 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

10886-1-AP targets Syndecan-3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Syndecan-3 fusion protein Ag1317 相同性解析による交差性が予測される生物種
Full Name	syndecan 3
Calculated molecular weight	38 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC013974
Gene Symbol	Syndecan-3
Gene ID (NCBI)	9672
RRID	AB_2182999
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75056
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Protocols

Product Specific Protocols
WB protocol for Syndecan-3 antibody 10886-1-AP	Download protocol
IHC protocol for Syndecan-3 antibody 10886-1-AP	Download protocol
IF protocol for Syndecan-3 antibody 10886-1-AP	Download protocol
IP protocol for Syndecan-3 antibody 10886-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Am J Pathol Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets. Authors - van Oosterwijk Jolieke G JG View Article
human	IHC	Am J Pathol No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas. Authors - Reijnders Christianne M A CM View Article
human	WB	J Cell Sci Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells. Authors - Yu-Ja Huang View Article
human	IHC	BMC Cancer Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma. Authors - Wu Zheng-Sheng ZS View Article
human	IHC	Medicine (Baltimore) Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3 Authors - Guozhi Yang View Article
mouse	IF	Int J Mol Sci GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway Authors - Apostolos Mikroulis View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10886-1-AP

WB analysis of L02 using 10886-1-AP

WB analysis of MCF-7 using 10886-1-AP

WB analysis of rat kidney using 10886-1-AP

WB analysis of Jurkat using 10886-1-AP

WB analysis of mouse kidney using 10886-1-AP

IP experiment of mouse lung using 10886-1-AP

IP experiment of A549 using 10886-1-AP

IHC staining of human colon cancer using 10886-1-AP

IHC staining of human colon cancer using 10886-1-AP

IF Staining of PC-12 using 10886-1-AP

IF Staining of MCF-7 using 10886-1-AP

Syndecan-3 Polyclonal antibody

Syndecan-3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.

No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.

Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3

GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway