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KD/KO Validated

Syntenin-1 Polyclonal antibody

Name: Syntenin-1 Polyclonal antibody
Brand: Proteintech
SKU: 22399-1-AP

Syntenin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22399-1-AP

Synonyms

MDA 9, MDA9, Scaffold protein Pbp1, SDCBP, ST1, SYCL, Syndecan binding protein 1, Syntenin-1, TACIP18

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 22399-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22399-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 22399-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 22399-1-AP
IP result of anti-Syntenin-1 (IP:22399-1-AP, 3ug; Detection:22399-1-AP 1:1000) with HeLa cells lysate 2500ug.
IHC staining of human liver cancer using 22399-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22399-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 22399-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 22399-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 22399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 22399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 22399-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Syntenin-1 antibody (22399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 22399-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, human placenta tissue, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, mouse brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 18 publications below
IHC	See 2 publications below

Product Information

22399-1-AP targets Syntenin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Syntenin-1 fusion protein Ag18078 相同性解析による交差性が予測される生物種
Full Name	syndecan binding protein (syntenin)
Calculated molecular weight	298 aa, 32 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC113674
Gene symbol	Syntenin-1
Gene ID (NCBI)	6386
RRID	AB_2879100
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.

Protocols

Product Specific Protocols
WB protocol for Syntenin-1 antibody 22399-1-AP	Download protocol
IHC protocol for Syntenin-1 antibody 22399-1-AP	Download protocol
IF protocol for Syntenin-1 antibody 22399-1-AP	Download protocol
IP protocol for Syntenin-1 antibody 22399-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res RAB31 marks and controls an ESCRT-independent exosome pathway. Authors - Denghui Wei KO Validated View Article
human,mouse	WB	Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity Authors - Ying Gao View Article
human,monkey	WB	EMBO J Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement Authors - Fanfan Zhao KD Validated View Article
human	WB	Cell Mol Life Sci Exosomes from TNF-α preconditioned human umbilical cord mesenchymal stromal cells inhibit the autophagy of acinar cells of severe acute pancreatitis via shuttling bioactive metabolites Authors - Zhilong Ma View Article
human	WB	Cell Oncol (Dordr) Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2. Authors - Ningye Ma View Article
human	WB	Cell Oncol (Dordr) EV-miRome-wide profiling uncovers miR-320c for detecting metastatic colorectal cancer and monitoring the therapeutic response. Authors - Yi-Tung Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntenin-1 Polyclonal antibody

Syntenin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 22399-1-AP

WB analysis using 22399-1-AP

WB analysis of human placenta using 22399-1-AP

IP experiment of HeLa using 22399-1-AP

IHC staining of human liver cancer using 22399-1-AP

IHC staining of human liver cancer using 22399-1-AP

IHC staining of human heart using 22399-1-AP

IHC staining of human heart using 22399-1-AP

IHC staining of mouse brain using 22399-1-AP

IHC staining of mouse brain using 22399-1-AP

IF Staining of HeLa using 22399-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RAB31 marks and controls an ESCRT-independent exosome pathway.

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement

Exosomes from TNF-α preconditioned human umbilical cord mesenchymal stromal cells inhibit the autophagy of acinar cells of severe acute pancreatitis via shuttling bioactive metabolites

Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2.

EV-miRome-wide profiling uncovers miR-320c for detecting metastatic colorectal cancer and monitoring the therapeutic response.