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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDF2 Polyclonal antibody (14747-1-AP)

    WB analysis using 14747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14747-1-AP (SDF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SDF2 Polyclonal antibody (14747-1-AP)

    WB analysis using 14747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14747-1-AP (SDF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SDF2 Polyclonal antibody (14747-1-AP)

    IHC staining of human ovary cancer using 14747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14747-1-AP (SDF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SDF2 Polyclonal antibody

SDF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14747-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Stromal cell-derived factor 2, stromal cell derived factor 2, SDF-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HUVEC cells, mouse heart, U2OS cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14747-1-AP targets SDF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDF2 fusion protein Ag6480 相同性解析による交差性が予測される生物種
Full Name stromal cell-derived factor 2
Calculated molecular weight 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC000500
Gene Symbol SDF2
Gene ID (NCBI) 6388
RRIDAB_2918037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99470
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDF-2 is a small protein of 211 amino acids that is ubiquitously expressed in lung, breast, colon, liver and kidney. SDF-2 is localized in the ER and has been reported to get involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control.

Protocols

Product Specific Protocols
WB protocol for SDF2 antibody 14747-1-APDownload protocol
IHC protocol for SDF2 antibody 14747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols