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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDHB Monoclonal antibody (67600-1-Ig)

    WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SDHB Monoclonal antibody (67600-1-Ig)

    WB analysis of HepG2 using 67600-1-Ig (same clone as 67600-1-PBS)

    WB result of SDHB antibody (67600-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SDHB transfected HepG2 cells. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SDHB Monoclonal antibody (67600-1-Ig)

    WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SDHB Monoclonal antibody (67600-1-Ig)

    WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SDHB Monoclonal antibody (67600-1-Ig)

    WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SDHB Monoclonal antibody (67600-1-Ig)

    IHC staining of human stomach cancer using 67600-1-Ig (same clone as 67600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SDHB Monoclonal antibody (67600-1-Ig)

    IHC staining of mouse skeletal muscle using 67600-1-Ig (same clone as 67600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SDHB Monoclonal antibody (67600-1-Ig)

    IHC staining of mouse skeletal muscle using 67600-1-Ig (same clone as 67600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SDHB Monoclonal antibody (67600-1-Ig)

    IF Staining of human liver cancer using 67600-1-Ig (same clone as 67600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SDHB antibody (67600-1-Ig, Clone: 1A2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SDHB Monoclonal antibody, PBS Only

SDHB Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67600-1-PBS
Clone No.1A2A5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

SDH, PGL4, PGL 4, Iron-sulfur subunit of complex II, EC:1.3.5.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67600-1-PBS targets SDHB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SDHB fusion protein Ag29868 相同性解析による交差性が予測される生物種
Full Name succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Calculated molecular weight 32 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC007840
Gene Symbol SDHB
Gene ID (NCBI) 6390
RRIDAB_2882806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21912
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).