SDHC Polyclonal antibody
SDHC Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14575-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 1 publications below |
Product Information
14575-1-AP targets SDHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SDHC fusion protein Ag5775 相同性解析による交差性が予測される生物種 |
| Full Name | succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC033626 |
| Gene symbol | SDHC |
| Gene ID (NCBI) | 6391 |
| RRID | AB_2183291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SDHC(Succinate dehydrogenase cytochrome b560 subunit, mitochondrial) is also named as CYB560, SDH3 and belongs to the cytochrome b560 family. It is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3) and paraganglioma and gastric stromal sarcoma (PGGSS). It has a transit peptide of 29 amino acid and has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SDHC antibody 14575-1-AP | Download protocol |
| IHC protocol for SDHC antibody 14575-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
Nucleic Acids Res Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice. | ||
iScience Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver. | ||
PLoS One Quantitative proteomics in A30P*A53T α-synuclein transgenic mice reveals upregulation of Sel1l. | ||
Cell Rep Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress | ||
Front Immunol Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma |
