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  • Featured Product
  • KD/KO Validated

SDPR Polyclonal antibody

SDPR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12339-1-AP

Synonyms

PS-p68, PS p68, Phosphatidylserine-binding protein, Cavin-2, Cavin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of A549 using 12339-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of mouse liver using 12339-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of mouse heart using 12339-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of mouse placenta using 12339-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of COLO 320 using 12339-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of mouse brain using 12339-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of A549 using 12339-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SDPR Polyclonal antibody (12339-1-AP)

    WB analysis of rat brain using 12339-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SDPR Polyclonal antibody (12339-1-AP)

    IP experiment of mouse heart using 12339-1-AP

    IP result of anti-SDPR (IP:12339-1-AP, 4ug; Detection:12339-1-AP 1:1000) with mouse heart tissue lysate 8000ug.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human renal cell carcinoma using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human normal colon using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human normal colon using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12339-1-AP (SDPR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human heart using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human heart using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human tonsillitis using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human tonsillitis using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human breast hyperplasia using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human breast hyperplasia using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human renal cell carcinoma using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human kidney using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SDPR Polyclonal antibody (12339-1-AP)

    IHC staining of human kidney using 12339-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SDPR Polyclonal antibody (12339-1-AP)

    IF Staining of A549 using 12339-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 12339-1-AP (SDPR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, mouse liver tissue, COLO 320 cells, mouse placenta tissue, rat brain tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human breast hyperplasia tissue, human heart tissue, human kidney tissue, human normal colon, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12339-1-AP targets SDPR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDPR fusion protein Ag2990 相同性解析による交差性が予測される生物種
Full Name serum deprivation response (phosphatidylserine binding protein)
Calculated molecular weight 425 aa, 47 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC016475
Gene symbol SDPR
Gene ID (NCBI) 8436
RRIDAB_2183305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serum deprivation-response protein(SDRP), a substrate of protein kinase C, is a member of a group of proteins that bind to phosphatidylserine in a clacium-dependent manner. SDPR may participate in the generation or stabilization of membrane curvature. SDPR is highly expressed in heart and lung, and low in brain,kidney, liver, pancreas, placenta and skeletal muscle. This is a rabbit polyclonal anti-SDPR antibody raised against C-terminus of human SDPR. SDRP of molecular masses 115 kDa and 100 kDa were found in neutrophils, monocytes, lymphocytes, platelets and erythrocytes. In platelets, however, the principal SDRP is a cytosolic protein of molecular mass 68 kDa. (PMID: 2390065 )

Protocols

Product Specific Protocols
WB protocol for SDPR antibody 12339-1-APDownload protocol
IHC protocol for SDPR antibody 12339-1-APDownload protocol
IF protocol for SDPR antibody 12339-1-APDownload protocol
IP protocol for SDPR antibody 12339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
  • KD Validated
View Article
mouse

Cardiovasc Res

Loss of Cavin-2 destabilizes PTEN and enhances Akt signaling pathway in cardiomyocytes

Authors - Naoki Maruyama
View Article
humanIF

Dev Cell

Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq.

Authors - Nicole D Ulrich
View Article
mouseWB

Haematologica

Dynamin 2 is required for GPVI signaling and platelet hemostatic function in mice.

Authors - Nathan Eaton
View Article
mouseWB,IF,IP

Mol Metab

Requirement of Cavin-2 for the expression and stability of IRβ in adequate adipocyte differentiation.

Authors - Yusuke Higuchi
View Article
ratIF

J Cereb Blood Flow Metab

P-glycoprotein traffics from the nucleus to the plasma membrane in rat brain endothelium during inflammatory pain.

Authors - Margaret E Tome
View Article