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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDS Polyclonal antibody (16594-1-AP)

    WB analysis using 16594-1-AP (same clone as 16594-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SDS Polyclonal antibody (16594-1-AP)

    WB analysis using 16594-1-AP (same clone as 16594-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SDS Polyclonal antibody (16594-1-AP)

    IHC staining of human liver using 16594-1-AP (same clone as 16594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SDS Polyclonal antibody (16594-1-AP)

    IHC staining of human liver using 16594-1-AP (same clone as 16594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

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SDS Polyclonal antibody, PBS Only

SDS Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16594-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16594-1-PBS targets SDS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDS fusion protein Ag9704 相同性解析による交差性が予測される生物種
Full Name serine dehydratase
Calculated molecular weight 218 aa, 23 kDa
Observed molecular weight35 kDa; 70 kDa
GenBank accession numberBC020750
Gene Symbol SDS
Gene ID (NCBI) 10993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20132
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

L-Serine dehydratase (SDS ; SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of l-serine to yield pyruvate. In mammals, SDH is found predominantly in the liver. Extensive studies have been carried out on SDH from rat and the enzyme has been found to play an important role in gluconeogenesis; its activity is induced by the consumption of high-protein diets, starvation and other treatments. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. (PMID: 25380533, PMID: 21878319, PMID: 14646100)