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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of HepG2 using 16242-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of human liver using 16242-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of human liver using 16242-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of human colon using 16242-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of mouse liver using 16242-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of mouse lung using 16242-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    IHC staining of mouse liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    IHC staining of mouse liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SDSL Polyclonal antibody (16242-1-AP)

    IF Staining of HepG2 using 16242-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SDSL antibody (16242-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SDSL Polyclonal antibody

SDSL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16242-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L threonine dehydratase, L serine deaminase, EC:4.3.1.19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16242-1-AP targets SDSL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDSL fusion protein Ag9114 相同性解析による交差性が予測される生物種
Full Name serine dehydratase-like
Calculated molecular weight 329 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC009849
Gene Symbol SDSL
Gene ID (NCBI) 113675
RRIDAB_2254163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SDSL antibody 16242-1-APDownload protocol
IHC protocol for SDSL antibody 16242-1-APDownload protocol
IF protocol for SDSL antibody 16242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols