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- KD/KO Validated
SEC11A Polyclonal antibody
SEC11A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14753-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, human colon tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
14753-1-AP targets SEC11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SEC11A fusion protein Ag6500 相同性解析による交差性が予測される生物種 |
| Full Name | SEC11 homolog A (S. cerevisiae) |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 17-21 kDa |
| GenBank accession number | BC000359 |
| Gene symbol | SEC11A |
| Gene ID (NCBI) | 23478 |
| RRID | AB_2186230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEC11A(Signal peptidase complex catalytic subunit SEC11A) is also named as SEC11L1, SPC18, SPCS4A and belongs to the peptidase S26B family. It is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. SEC11A has some isoforms with the molecular mass of 17-21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC11A antibody 14753-1-AP | Download protocol |
| IHC protocol for SEC11A antibody 14753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.
| ||
Cell Res Switching off IMMP2L signaling drives senescence via simultaneous metabolic alteration and blockage of cell death. | ||
Mol Cell Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway |
