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  • Featured Product
  • KD/KO Validated

SEC14L1 Polyclonal antibody

SEC14L1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25541-1-AP

Synonyms

SEC14-like protein 1, SEC14L

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using SEC14L1 Polyclonal antibody (25541-1-AP)

    WB analysis of Y79 using 25541-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SEC14L1 Polyclonal antibody (25541-1-AP)

    WB analysis using 25541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SEC14L1 Polyclonal antibody (25541-1-AP)

    IP experiment of HEK-293 using 25541-1-AP

    IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:500) with HEK-293 cells lysate 1480 ug.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SEC14L1 Polyclonal antibody (25541-1-AP)

    IP experiment of HEK-293 using 25541-1-AP

    IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SEC14L1 Polyclonal antibody (25541-1-AP)

    IF Staining of A431 using 25541-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SEC14L1 antibody (25541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25541-1-AP targets SEC14L1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC14L1 fusion protein Ag22233 相同性解析による交差性が予測される生物種
Full Name SEC14-like 1 (S. cerevisiae)
Calculated molecular weight 719 aa, 82 kDa
Observed molecular weight 81 kDa, 77 kDa
GenBank accession numberBC142979
Gene symbol SEC14L1
Gene ID (NCBI) 6397
RRIDAB_2880125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC14L1 (SEC14-like protein 1) is a member of SEC14 family in mammals with the potential to control local phospholipid content in membranes. SEC14L1 may act as a phospholipid transfer protein. It has also been reported that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling and is involved in innate immunity (PMID: 17092608; 23843640). SEC14L1 has three isoforms with the molecular mass of 81.2kDa, 81.8kDa and 77.1kDa.

Protocols

Product Specific Protocols
WB protocol for SEC14L1 antibody 25541-1-APDownload protocol
IF protocol for SEC14L1 antibody 25541-1-APDownload protocol
IP protocol for SEC14L1 antibody 25541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

Authors - Birol Cabukusta
  • KO Validated
View Article