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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC16A Polyclonal antibody

SEC16A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 29417-1-AP

Synonyms

SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC16A Polyclonal antibody (29417-1-AP)

    WB analysis using 29417-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29417-1-AP (SEC16A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SEC16A Polyclonal antibody (29417-1-AP)

    WB analysis using 29417-1-AP

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 29417-1-AP (SEC16A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC16A Polyclonal antibody (29417-1-AP)

    IHC staining of mouse pancreas using 29417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29417-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC16A Polyclonal antibody (29417-1-AP)

    IF Staining of HeLa using 29417-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC16A antibody (29417-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SEC16A Polyclonal antibody (29417-1-AP)

    FC experiment of HeLa using 29417-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (29417-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29417-1-AP targets SEC16A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC16A fusion protein Ag30700 相同性解析による交差性が予測される生物種
Full Name SEC16 homolog A (S. cerevisiae)
Calculated molecular weight 252 kDa
Observed molecular weight 250-300 kDa
GenBank accession numberNM_014866
Gene symbol SEC16A
Gene ID (NCBI) 9919
RRIDAB_3086130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

Protocols

Product Specific Protocols
WB protocol for SEC16A antibody 29417-1-APDownload protocol
IHC protocol for SEC16A antibody 29417-1-APDownload protocol
IF protocol for SEC16A antibody 29417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols