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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC16B Polyclonal antibody (17245-1-AP)

    WB analysis using 17245-1-AP

    mouse pancreas tissue was subjected to SDS PAGE followed by western blot with 17245-1-AP (SEC16B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SEC16B Polyclonal antibody (17245-1-AP)

    IHC staining of human pancreas cancer using 17245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17245-1-AP (SEC16B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SEC16B Polyclonal antibody

SEC16B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17245-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1928, LZTR2, PGPR p117, RGPR, RGPR p117, SEC16 homolog B, SEC16B, SEC16S

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17245-1-AP targets SEC16B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC16B fusion protein Ag11173 相同性解析による交差性が予測される生物種
Full Name SEC16 homolog B (S. cerevisiae)
Calculated molecular weight 71 kDa, 117 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC009106
Gene Symbol SEC16B
Gene ID (NCBI) 89866
RRIDAB_3085519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC16B also known as LZTR2, RGPR-p117, is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (PMID: 17192411). SEC16B regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes (PMID:21768384). SEC16B may be involved in human obesity to possess an association between single nucleotide polymorphisms and different measures of obesity (PMID: 28413634).

Protocols

Product Specific Protocols
WB protocol for SEC16B antibody 17245-1-APDownload protocol
IHC protocol for SEC16B antibody 17245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols