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  • KD/KO Validated

SEC31A Polyclonal antibody

SEC31A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17913-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, IP, ELISA

SEC31L1, SEC31 related protein A, SEC31 like protein 1, KIAA0905, ABP130

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17913-1-AP targets SEC31A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC31A fusion protein Ag12314 相同性解析による交差性が予測される生物種
Full Name SEC31 homolog A (S. cerevisiae)
Calculated molecular weight 1220 aa, 133 kDa
Observed molecular weight 118-134 kDa
GenBank accession numberBC084583
Gene Symbol SEC31A
Gene ID (NCBI) 22872
RRIDAB_2186378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94979
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Protocols

Product Specific Protocols
WB protocol for SEC31A antibody 17913-1-APDownload protocol
IF protocol for SEC31A antibody 17913-1-APDownload protocol
IP protocol for SEC31A antibody 17913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP,WB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
  • KO Validated
mouseIF

Cell

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Authors - Moran Dvela-Levitt
mouseIF

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
humanIF

Dev Cell

SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.

Authors - Rui Yan
human,mouseWB

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
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