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  • Featured Product
  • KD/KO Validated

SEC5/EXOC2 Polyclonal antibody, PBS Only

SEC5/EXOC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12751-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SEC5/EXOC2 Polyclonal antibody (12751-1-AP)

    WB analysis of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SEC5/EXOC2 Polyclonal antibody (12751-1-AP)

    WB analysis of human brain using 12751-1-AP (same clone as 12751-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ileum tissue using SEC5/EXOC2 Polyclonal antibody (12751-1-AP)

    WB analysis of human ileum using 12751-1-AP (same clone as 12751-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEC5/EXOC2 Polyclonal antibody (12751-1-AP)

    IP experiment of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

    IP result of anti-SEC5/EXOC2 (IP:12751-1-AP, 5ug; Detection:12751-1-AP 1:500) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SEC5/EXOC2 Polyclonal antibody (12751-1-AP)

    IHC staining of human breast cancer using 12751-1-AP (same clone as 12751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC5/EXOC2 Polyclonal antibody (12751-1-AP)

    IF Staining of HeLa using 12751-1-AP (same clone as 12751-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12751-1-PBS targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC5/EXOC2 fusion protein Ag3147 相同性解析による交差性が予測される生物種
Full Name exocyst complex component 2
Calculated molecular weight 924 aa, 104 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC016918
Gene symbol SEC5
Gene ID (NCBI) 55770
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.