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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC61B-Specific Polyclonal antibody, PBS Only

SEC61B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14846-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    WB analysis using 14846-1-AP (same clone as 14846-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    WB analysis of HeLa using 14846-1-AP (same clone as 14846-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    WB analysis of human kidney using 14846-1-AP (same clone as 14846-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IHC staining of human cervical cancer using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IHC staining of human cervical cancer using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IHC staining of human liver using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IHC staining of human liver using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC61B-Specific Polyclonal antibody (14846-1-AP)

    IF Staining of HeLa using 14846-1-AP (same clone as 14846-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14846-1-PBS targets SEC61B-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberNM_006808
Gene symbol SEC61B
Gene ID (NCBI) 10952
RRIDAB_2270168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).