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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC62 Polyclonal antibody

SEC62 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28693-1-AP

Synonyms

Translocation protein SEC62, Translocation protein 1, TP-1, SEC 62, hTP-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC62 Polyclonal antibody (28693-1-AP)

    WB analysis using 28693-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28693-1-AP (SEC62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC62 Polyclonal antibody (28693-1-AP)

    IF Staining of HepG2 using 28693-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC62 antibody (28693-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHT-29 cells, SW480 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28693-1-AP targets SEC62 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC62 fusion protein Ag29932 相同性解析による交差性が予測される生物種
Full Name SEC62 homolog (S. cerevisiae)
Calculated molecular weight 46 kDa
Observed molecular weight52 kDa
GenBank accession numberNM_003262
Gene symbol SEC62
Gene ID (NCBI) 7095
RRIDAB_3086078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC62 is a integral endoplasmic reticulum (ER) membrane protein. SEC62 and SEC63 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. SEC62 is associated with poor prognosis in a variety of tumors, such as prostate cancer, hepatocellular cancer, breast cancer , melanoma, colorectal cancer (PMID: 36262254).

Protocols

Product Specific Protocols
WB protocol for SEC62 antibody 28693-1-APDownload protocol
IF protocol for SEC62 antibody 28693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

ER-phagy restrains inflammatory responses through its receptor UBAC2

Authors - Xing He
View Article