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  • Featured Product
  • KD/KO Validated

SEC63 Polyclonal antibody

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 13978-1-AP

Synonyms

Translocation protein SEC63 homolog, SEC63L, DNAJC23, DnaJ homolog subfamily C member 23

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using SEC63 Polyclonal antibody (13978-1-AP)

    WB analysis of A431 using 13978-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SEC63 Polyclonal antibody (13978-1-AP)

    WB analysis of PC-3 using 13978-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SEC63 Polyclonal antibody (13978-1-AP)

    WB analysis of HEK-293 using 13978-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using SEC63 Polyclonal antibody (13978-1-AP)

    IP experiment of A431 using 13978-1-AP

    IP result of anti-SEC63 (IP:13978-1-AP, 4ug; Detection:13978-1-AP 1:300) with A431 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC63 Polyclonal antibody (13978-1-AP)

    IHC staining of human liver using 13978-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC63 Polyclonal antibody (13978-1-AP)

    IHC staining of human liver using 13978-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC63 Polyclonal antibody (13978-1-AP)

    IHC staining of mouse pancreas using 13978-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC63 Polyclonal antibody (13978-1-AP)

    IHC staining of mouse pancreas using 13978-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using SEC63 Polyclonal antibody (13978-1-AP)

    IF Staining of mouse kidney using 13978-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using SEC63 Polyclonal antibody (13978-1-AP)

    IF Staining of mouse kidney using 13978-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEC63 Polyclonal antibody (13978-1-AP)

    IF Staining of HEK-293 using 13978-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (13978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, PC-3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman liver tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13978-1-AP targets SEC63 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC63 fusion protein Ag5021 相同性解析による交差性が予測される生物種
Full Name SEC63 homolog (S. cerevisiae)
Calculated molecular weight 88 kDa
Observed molecular weight 83-90 kDa
GenBank accession numberBC047221
Gene symbol SEC63
Gene ID (NCBI) 11231
RRIDAB_2186546
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC63 is an integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in the translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

Protocols

Product Specific Protocols
WB protocol for SEC63 antibody 13978-1-APDownload protocol
IHC protocol for SEC63 antibody 13978-1-APDownload protocol
IF protocol for SEC63 antibody 13978-1-APDownload protocol
IP protocol for SEC63 antibody 13978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Cell

Cotranslational Stabilization of Sec62/63 within the ER Sec61 Translocon Is Controlled by Distinct Substrate-Driven Translocation Events.

Authors - Brian J Conti
View Article
mouseWB

J Clin Invest

Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.

Authors - Sorin V Fedeles
View Article
mouseWB, IF

J Am Soc Nephrol

Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.

Authors - Hossam Hassan
  • KO Validated
View Article
humanWB

J Biol Chem

ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence

Authors - Tetsuya Hirata
  • KO Validated
View Article