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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC63 Polyclonal antibody

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28665-1-AP

Synonyms

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC63 Polyclonal antibody (28665-1-AP)

    WB analysis using 28665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28665-1-AP (SEC63 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of mouse pancreas using 28665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of mouse pancreas using 28665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of human liver using 28665-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of human liver using 28665-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEC63 Polyclonal antibody (28665-1-AP)

    IF Staining of HEK-293 using 28665-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (28665-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inmouse pancreas tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28665-1-AP targets SEC63 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC63 fusion protein Ag30213 相同性解析による交差性が予測される生物種
Full Name SEC63 homolog (S. cerevisiae)
Calculated molecular weight 88 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC047221
Gene symbol SEC63
Gene ID (NCBI) 11231
RRIDAB_2881189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

Protocols

Product Specific Protocols
WB protocol for SEC63 antibody 28665-1-APDownload protocol
IHC protocol for SEC63 antibody 28665-1-APDownload protocol
IF protocol for SEC63 antibody 28665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols