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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC63 Monoclonal antibody (67352-1-Ig)

    WB analysis using 67352-1-Ig (same clone as 67352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67352-1-Ig (SEC63 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEC63 Monoclonal antibody (67352-1-Ig)

    IF Staining of HEK-293 using 67352-1-Ig (same clone as 67352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SEC63 antibody (67352-1-Ig, Clone: 1C10E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

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SEC63 Monoclonal antibody, PBS Only

SEC63 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67352-1-PBS
Clone No.1C10E1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67352-1-PBS targets SEC63 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SEC63 fusion protein Ag5783 相同性解析による交差性が予測される生物種
Full Name SEC63 homolog (S. cerevisiae)
Calculated molecular weight 88 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC047221
Gene Symbol SEC63
Gene ID (NCBI) 11231
RRIDAB_2882608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UGP8
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)