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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEL1L Polyclonal antibody

SEL1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29801-1-AP

Synonyms

UNQ128/PRO1063, Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEL1L Polyclonal antibody (29801-1-AP)

    WB analysis using 29801-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29801-1-AP (SEL1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SEL1L Polyclonal antibody (29801-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SEL1L Polyclonal antibody (29801-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SEL1L Polyclonal antibody (29801-1-AP)

    IHC staining of human ovary cancer using 29801-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SEL1L Polyclonal antibody (29801-1-AP)

    IHC staining of human ovary cancer using 29801-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, BXPC-3 cells, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29801-1-AP targets SEL1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEL1L fusion protein Ag32178 相同性解析による交差性が予測される生物種
Full Name sel-1 suppressor of lin-12-like (C. elegans)
Calculated molecular weight 89 kDa
Observed molecular weight89 kDa
GenBank accession numberNM_005065
Gene symbol SEL1L
Gene ID (NCBI) 6400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen.

Protocols

Product Specific Protocols
WB protocol for SEL1L antibody 29801-1-APDownload protocol
IHC protocol for SEL1L antibody 29801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols