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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEL1L Recombinant antibody (84317-7-RR)

    WB analysis using 84317-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using SEL1L Recombinant antibody (84317-7-RR)

    FC experiment of MCF-7 using 84317-7-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SEL1L Recombinant antibody (84317-7-RR, Clone:241594G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84317-7-RR

    Affinity and Kinetic Characterization of 84317-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84317-7-RR against Human SEL1L were performed. The affinity constant is 6.03 nM.

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SEL1L Recombinant antibody

SEL1L Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84317-7-RR
Clone No.241594G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1, Protein sel 1 homolog 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, BxPC-3 cells, U-87 MG cells, NIH/3T3 cells, mouse kidney tissue, rat liver tissue
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84317-7-RR targets SEL1L in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEL1L fusion protein Ag32178 相同性解析による交差性が予測される生物種
Full Name sel-1 suppressor of lin-12-like (C. elegans)
Calculated molecular weight 89 kDa
Observed molecular weight89 kDa
GenBank accession numberNM_005065
Gene Symbol SEL1L
Gene ID (NCBI) 6400
RRIDAB_3671858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .

Protocols

Product Specific Protocols
WB protocol for SEL1L antibody 84317-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols