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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SELENBP1 Polyclonal antibody (27449-1-AP)

    WB analysis using 27449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SELENBP1 Polyclonal antibody (27449-1-AP)

    WB analysis of HEK-293 using 27449-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SELENBP1 Polyclonal antibody (27449-1-AP)

    WB analysis of mouse liver using 27449-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SELENBP1 Polyclonal antibody (27449-1-AP)

    IHC staining of human liver cancer using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SELENBP1 Polyclonal antibody (27449-1-AP)

    IHC staining of human liver cancer using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using SELENBP1 Polyclonal antibody (27449-1-AP)

    IHC staining of rat liver using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using SELENBP1 Polyclonal antibody (27449-1-AP)

    IHC staining of rat liver using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SELENBP1 Polyclonal antibody (27449-1-AP)

    IHC staining of human colon using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SELENBP1 Polyclonal antibody

SELENBP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27449-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Methanethiol oxidase, LPSB, hSP56, hSBP, EC:1.8.3.4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27449-1-AP targets SELENBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SELENBP1 fusion protein Ag24453 相同性解析による交差性が予測される生物種
Full Name selenium binding protein 1
Calculated molecular weight 52 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC009084
Gene Symbol SELENBP1
Gene ID (NCBI) 8991
RRIDAB_2880872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13228
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SELENBP1 (selenium binding protein 1,SBP1), also known as LPSB or SP56, is a 472 amino acid peripheral membrane protein that binds selenium covalently. Selenium, an essential trace element, has been shown to have an anti-cancer effect. Many reports have described a relationship between insufficient selenium intake and increased risk of cancer. Moreover, the expression of SELENBP1 has been shown to be decreased in several tumors including cancers of the prostate, lungs, colon, and ovary (PMID: 21143902, 20332323, 18272210). SELENBP1 has four isoforms with the molecular mass of 56kD, 52kD and 45kD.

Protocols

Product Specific Protocols
WB protocol for SELENBP1 antibody 27449-1-APDownload protocol
IHC protocol for SELENBP1 antibody 27449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols