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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SELPLG/CD162 Rabbit Recombinant Antibod (98084-1-RR)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10), or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98084-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SELPLG/CD162 Rabbit Recombinant Antibod (98084-1-RR)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) (red), or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98084-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SELPLG/CD162 Rabbit Recombinant Antibod (98084-1-RR)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR (same clone as 98084-1-PBS)

    1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98084-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98084-1-RR

    Affinity and Kinetic Characterization of 98084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98084-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.699 nM.

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Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody, PBS Only

SELPLG/CD162 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98084-1-PBS
Clone No.241297F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98084-1-PBS targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name selectin P ligand
Calculated molecular weight 412 aa, 43 kDa
GenBank accession numberBC029782
Gene Symbol SELPLG
Gene ID (NCBI) 6404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14242
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).