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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SELPLG/CD162 Recombinant antibody, PBS Only

SELPLG/CD162 Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241297G10

Cat no : 84500-1-PBS

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using SELPLG/CD162 Recombinant antibody (84500-1-RR)

    IF Staining of Jurkat using 84500-1-RR (same clone as 84500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SELPLG antibody (84500-1-RR, Clone: 241297G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84500-1-RR

    Affinity and Kinetic Characterization of 84500-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84500-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.221 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84500-1-PBS targets SELPLG/CD162 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name selectin P ligand
Calculated molecular weight 412 aa, 43 kDa
GenBank accession numberBC029782
Gene symbol SELPLG
Gene ID (NCBI) 6404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).