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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEMA3A Recombinant antibody, PBS Only

SEMA3A Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240509E3

Cat no : 83579-5-PBS

Synonyms

SEMA1, Sema III, Hsema III, Hsema I, coll 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA3A Recombinant antibody (83579-5-RR)

    WB analysis using 83579-5-RR (same clone as 83579-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83579-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83579-5-RR

    Affinity and Kinetic Characterization of 83579-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83579-5-PBS is SEMA3A Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEMA3A fusion protein Ag27173 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Calculated molecular weight 771 aa, 89 kDa
GenBank accession numberBC111416
Gene symbol SEMA3A
Gene ID (NCBI) 10371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.