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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SEMA4B Polyclonal antibody (29191-1-AP)

    IHC staining of human colon cancer using 29191-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29191-1-AP (SEMA4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SEMA4B Polyclonal antibody (29191-1-AP)

    IHC staining of human colon cancer using 29191-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29191-1-AP (SEMA4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SEMA4B Polyclonal antibody

SEMA4B Polyclonal Antibody for IHC, ELISA
Cat No. 29191-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Synonyms

KIAA1745, SEMA4B, SEMAC, SemC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29191-1-AP targets SEMA4B in IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEMA4B fusion protein Ag30422 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
GenBank accession numberBC110853
Gene Symbol SEMA4B
Gene ID (NCBI) 10509
RRIDAB_3086102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEMA4B (Semaphorin 4B), belonging to the Class SEMA4, is a transmembrane homodimer glycoprotein. Lots of SEMA4 family members have been implicated in the formation and progression of tumors due to (PMID: 35676688). SEMA4B inhibits the invasion of non-small cell lung cancer (NSCLC) through PI3K-dependent suppression of MMP9 activation (PMID: 25746385).

Protocols

Product Specific Protocols
IHC protocol for SEMA4B antibody 29191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols