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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEMA4C Polyclonal antibody, PBS Only

SEMA4C Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28402-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA4C Polyclonal antibody (28402-1-AP)

    WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SEMA4C Polyclonal antibody (28402-1-AP)

    WB analysis of mouse brain using 28402-1-AP (same clone as 28402-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SEMA4C Polyclonal antibody (28402-1-AP)

    WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

    Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SEMA4C Polyclonal antibody (28402-1-AP)

    IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SEMA4C Polyclonal antibody (28402-1-AP)

    IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SEMA4C Polyclonal antibody (28402-1-AP)

    IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SEMA4C Polyclonal antibody (28402-1-AP)

    IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28402-1-PBS targets SEMA4C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEMA4C fusion protein Ag29143 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
Calculated molecular weight 833 aa, 93 kDa
Observed molecular weight93 kDa
GenBank accession numberBC109104
Gene symbol SEMA4C
Gene ID (NCBI) 54910
RRIDAB_2881134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).