Validation Data Gallery
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Tested Applications
| Positive WB detected in | human placenta tissue, mouse brain tissue |
| Positive IP detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IP | See 1 publications below |
Product Information
18070-1-AP targets SEMA7A in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SEMA7A fusion protein Ag12707 相同性解析による交差性が予測される生物種 |
| Full Name | semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) |
| Calculated molecular weight | 666 aa, 75 kDa |
| Observed molecular weight | 75-80 kDa |
| GenBank accession number | BC101643 |
| Gene Symbol | SEMA7A |
| Gene ID (NCBI) | 8482 |
| RRID | AB_2878489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75326 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA7A antibody 18070-1-AP | Download protocol |
| IP protocol for SEMA7A antibody 18070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer | ||
Mol Metab Sema7A protects against high-fat diet-induced obesity and hepatic steatosis by regulating adipo/lipogenesis | ||
J Cancer NCAPG promotes the malignant progression of endometrioid cancer through LEF1/SEMA7A/PI3K-AKT |