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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SENP1 Recombinant antibody

SENP1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240478F3

Cat no : 83594-1-RR

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478F3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SENP1 Recombinant antibody (83594-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83594-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83594-1-RR (SENP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SENP1 Recombinant antibody (83594-1-RR)

    IF Staining of A431 using 83594-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP1 antibody (83594-1-RR, Clone: 240478F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SENP1 Recombinant antibody (83594-1-RR)

    FC experiment of HeLa using 83594-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SENP1 Recombinant antibody (83594-1-RR, Clone:240478F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83594-1-RR

    Affinity and Kinetic Characterization of 83594-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-1-RR against Human SENP1 were performed. The affinity constant is 1.91 nM.

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Tested Applications

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83594-1-RR is SENP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SENP1 fusion protein Ag18028 相同性解析による交差性が予測される生物種
Full Name SUMO1/sentrin specific peptidase 1
Calculated molecular weight 644 aa, 73 kDa
GenBank accession numberBC045639
Gene symbol SENP1
Gene ID (NCBI) 29843
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Protocols

Product Specific Protocols
IHC protocol for SENP1 antibody 83594-1-RRDownload protocol
IF protocol for SENP1 antibody 83594-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols