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  • KD/KO Validated

SENP3 Polyclonal antibody

SENP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 17659-1-AP

Synonyms

SUMO-1-specific protease 3, SSP3, SMT3IP1, Sentrin-specific protease 3, Sentrin/SUMO-specific protease SENP3


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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Y79 cells, rat embryo tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17659-1-AP targets SENP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SENP3 fusion protein Ag11911 相同性解析による交差性が予測される生物種
Full Name SUMO1/sentrin/SMT3 specific peptidase 3
Calculated molecular weight 574 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC080658
Gene symbol SENP3
Gene ID (NCBI) 26168
RRIDAB_2301618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.

Protocols

Product Specific Protocols
WB protocol for SENP3 antibody 17659-1-APDownload protocol
IHC protocol for SENP3 antibody 17659-1-APDownload protocol
IP protocol for SENP3 antibody 17659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.

Authors - Fanis Pavlos P
humanWB,IF

FASEB J

SUMO1 SUMOylates and SENP3 deSUMOylates NLRP3 to orchestrate the inflammasome activation.

Authors - Luyao Shao
humanWB

Breast Cancer Res Treat

Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34

Authors - Kristin A Altwegg
mouseWB,IF

iScience

SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury

Authors - Qian Xia
  • KD Validated
humanIP,WB

Mol Oncol

PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

Authors - Xue Yang
human,ratWB

CNS Neurosci Ther

Astragaloside IV combined with ligustrazine ameliorates abnormal mitochondrial dynamics via Drp1 SUMO/deSUMOylation in cerebral ischemia-reperfusion injury

Authors - Xiangyu Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons