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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

SENP8 Polyclonal antibody

SENP8 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13479-1-AP

Synonyms

HsT17512, FKSG8, EC:3.4.22.-, Deneddylase-1, Deneddylase 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of human colon cancer using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of human kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of human stomach cancer using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of mouse pancreas using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of rat kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of rat pancreas using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of mouse kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of mouse kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SENP8 Polyclonal antibody (13479-1-AP)

    IHC staining of human liver using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SENP8 Polyclonal antibody (13479-1-AP)

    IF Staining of A431 using 13479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP8 antibody (13479-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive IHC detected inrat kidney tissue, human liver tissue, human colon cancer tissue, human kidney tissue, human stomach cancer tissue, mouse pancreas tissue, rat pancreas tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13479-1-AP targets SENP8 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SENP8 fusion protein Ag4287 相同性解析による交差性が予測される生物種
Full Name SUMO/sentrin specific peptidase family member 8
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight24-28 kDa
GenBank accession numberBC031411
Gene symbol SENP8
Gene ID (NCBI) 123228
RRIDAB_3085415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SENP8, a human NEDD8-specific protease, also known as NEDP1. It is expected to be located in cytoplasm and nucleus, which is highly expressed in kidney and pancreas. The calculated molecular weight of SENP8 is 24 kDa. It is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53 (PMID: 15242646). A single-residue difference in the C-terminus of NEDD8 and ubiquitin contributes significantly to the ability of NEDP1 to discriminate between them. In vivo analysis indicates that NEDP1 mutants perturb deNEDDylation of the tumour suppressor p53 (PMID: 12759363).

Protocols

Product Specific Protocols
IHC protocol for SENP8 antibody 13479-1-APDownload protocol
IF protocol for SENP8 antibody 13479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols