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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

SEPHS1 Polyclonal antibody

SEPHS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16635-1-AP

Synonyms

SELD, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1, selenophosphate synthetase 1, SEPHS1, SPS, SPS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPHS1 Polyclonal antibody (16635-1-AP)

    WB analysis using 16635-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SEPHS1 Polyclonal antibody (16635-1-AP)

    WB analysis of HepG2 using 16635-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SEPHS1 Polyclonal antibody (16635-1-AP)

    WB analysis of mouse liver using 16635-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SEPHS1 Polyclonal antibody (16635-1-AP)

    IHC staining of human lung cancer using 16635-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SEPHS1 Polyclonal antibody (16635-1-AP)

    IHC staining of human lung cancer using 16635-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, mouse liver tissue, T-47D cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16635-1-AP targets SEPHS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEPHS1 fusion protein Ag9978 相同性解析による交差性が予測される生物種
Full Name selenophosphate synthetase 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC000941
Gene symbol SEPHS1
Gene ID (NCBI) 22929
RRIDAB_10603472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Selenophosphate synthetase 1 (SEPHS1) has been implicated in the maintenance of redox homeostasis in chondrocytes and its deficiency plays a causal role in the progression of osteoarthritis (PMID: 36202216). SEPHS1 downregulation in human umbilical vein cells (HUVECs) led to cell death rather than the inhibition of cell proliferation (PMID: 33622411).

Protocols

Product Specific Protocols
WB protocol for SEPHS1 antibody 16635-1-APDownload protocol
IHC protocol for SEPHS1 antibody 16635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biomaterials

H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence

Authors - Fan Yang
View Article
humanWB,IHC

Exp Hematol Oncol

SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.

Authors - Shu Yang
  • KD Validated
View Article