SEPHS2 Polyclonal antibody

Name: SEPHS2 Polyclonal antibody
Brand: Proteintech
SKU: 14109-1-AP

SEPHS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14109-1-AP

Synonyms

Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, selenophosphate synthetase 2, SEPHS2, SPS2

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 14109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 14109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 14109-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 14109-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

-/ -

Cat No. 14109-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NCI-H1299 cells, mouse pancreas tissue, mouse adipose tissue, rat liver tissue
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

14109-1-AP targets SEPHS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SEPHS2 fusion protein Ag5255 相同性解析による交差性が予測される生物種
Full Name	selenophosphate synthetase 2
Calculated molecular weight	47 kDa
Observed molecular weight	47-50 kDa
GenBank accession number	BC016643
Gene symbol	SEPHS2
Gene ID (NCBI)	22928
RRID	AB_2878014
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SEPHS2 antibody 14109-1-AP	Download protocol
IHC protocol for SEPHS2 antibody 14109-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Biomaterials H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence Authors - Fan Yang View Article
human	WB	EMBO Mol Med Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis Authors - Tobias Ackermann View Article
human	IHC	Front Med (Lausanne) A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma Authors - Fengyan Wang View Article
human,mouse	WB	Nat Struct Mol Biol PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis Authors - Hiroaki Fujita View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEPHS2 Polyclonal antibody

SEPHS2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 14109-1-AP

WB analysis using 14109-1-AP

IHC staining of human breast cancer using 14109-1-AP

IHC staining of human liver cancer using 14109-1-AP

IHC staining of mouse pancreas using 14109-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence

Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis

A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma

PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis