Septin 11 Polyclonal antibody

Name: Septin 11 Polyclonal antibody
Brand: Proteintech
SKU: 14672-1-AP

Septin 11 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14672-1-AP

Synonyms

SEPTIN11, SEPT11, Septin-11

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of human brain using 14672-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 14672-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 14672-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14672-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14672-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 14672-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 14672-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14672-1-AP
IP result of anti-Septin 11 (IP:14672-1-AP, 4ug; Detection:14672-1-AP 1:500) with mouse brain tissue lysate 3440ug.
IHC staining of human ovary using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 14672-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 14672-1-AP
Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 14672-1-AP
Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 14672-1-AP
Immunofluorescent analysis of HepG2 cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of HeLa using 14672-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Septin 11 (14672-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 14672-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, HeLa cells, HT-1080 cells, mouse brain tissue, mouse colon tissue, mouse small intestine tissue, SH-SY5Y cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human ovary tissue, human kidney tissue, human lung tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

14672-1-AP targets Septin 11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Septin 11 fusion protein Ag6342 相同性解析による交差性が予測される生物種
Full Name	septin 11
Calculated molecular weight	49 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC063615
Gene symbol	Septin 11
Gene ID (NCBI)	55752
RRID	AB_2301682
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Septin 11 antibody 14672-1-AP	Download protocol
IHC protocol for Septin 11 antibody 14672-1-AP	Download protocol
IF protocol for Septin 11 antibody 14672-1-AP	Download protocol
IP protocol for Septin 11 antibody 14672-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	RIP	Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. Authors - Chi Hin Wong View Article
mouse	IF	Cytoskeleton (Hoboken) Characterization of septin expression in normal and fibrotic kidneys. Authors - Katharina Neubauer View Article
human	IF,WB	Cell Rep An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis Authors - Thomas C Panagiotou View Article
human	WB	Cancers (Basel) Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer Authors - Kyukwang Kim View Article
human,mouse	WB,IP,IF,CoIP	Cell Death Dis Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion Authors - Lisheng Fu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Septin 11 Polyclonal antibody

Septin 11 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of human brain using 14672-1-AP

WB analysis of mouse colon using 14672-1-AP

WB analysis of SH-SY5Y using 14672-1-AP

WB analysis of mouse brain using 14672-1-AP

WB analysis of HeLa using 14672-1-AP

WB analysis of HT-1080 using 14672-1-AP

WB analysis of mouse small intestine using 14672-1-AP

IP experiment of mouse brain using 14672-1-AP

IHC staining of human ovary using 14672-1-AP

IHC staining of human ovary using 14672-1-AP

IHC staining of human kidney using 14672-1-AP

IHC staining of human kidney using 14672-1-AP

IHC staining of human spleen using 14672-1-AP

IHC staining of human spleen using 14672-1-AP

IHC staining of human lung using 14672-1-AP

IHC staining of human lung using 14672-1-AP

IF Staining of HeLa using 14672-1-AP

IF Staining of HeLa using 14672-1-AP

IF Staining of HepG2 using 14672-1-AP

FC experiment of HeLa using 14672-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.

Characterization of septin expression in normal and fibrotic kidneys.

An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis

Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer

Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion