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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT3 Polyclonal antibody (30146-1-AP)

    WB analysis using 30146-1-AP (same clone as 30146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30146-1-AP (SEPT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEPT3 Polyclonal antibody (30146-1-AP)

    IP experiment of mouse brain using 30146-1-AP (same clone as 30146-1-PBS)

    IP result of anti-SEPT3 (IP:30146-1-AP, 4ug; Detection:30146-1-AP 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEPT3 Polyclonal antibody (30146-1-AP)

    IHC staining of mouse brain using 30146-1-AP (same clone as 30146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SEPT3 Polyclonal antibody (30146-1-AP)

    IHC staining of rat brain using 30146-1-AP (same clone as 30146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using SEPT3 Polyclonal antibody (30146-1-AP)

    FC experiment of HEK-293 using 30146-1-AP (same clone as 30146-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEPT3 (30146-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

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SEPT3 Polyclonal antibody, PBS Only

SEPT3 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 30146-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30146-1-PBS targets SEPT3 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEPT3 fusion protein Ag32890 相同性解析による交差性が予測される生物種
Full Name septin 3
Calculated molecular weight 358 aa, 41 kDa
Observed molecular weight40 kDa
GenBank accession numberBC111779
Gene Symbol SEPT3
Gene ID (NCBI) 55964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UH03
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SEPT3 is a developmentally regulated phosphoprotein. SEPT3 is a member of the septin GTPase family, which can form polymers and contribute to the cytoskeleton. SEPT3 is involved in neuronal autophagy. In the process of autophagy regulation, the level of SEPT3 will change according to the autophagy protein. SEPT3 is specifically expressed in the brain (PMID: 15485489; 35932293).