Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A375 cells, rat brain tissue, mouse cerebellum tissue |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
12476-1-AP targets Septin 4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Septin 4 fusion protein Ag3185 相同性解析による交差性が予測される生物種 |
| Full Name | septin 4 |
| Calculated molecular weight | 459 aa, 53 kDa |
| Observed molecular weight | 54 kDa, 52 kDa, 48 kDa, 44 kDa |
| GenBank accession number | BC018056 |
| Gene Symbol | SEPT4 |
| Gene ID (NCBI) | 5414 |
| RRID | AB_2187151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| IHC protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| IF protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| IP protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioorg Chem Discovery of a septin-4 covalent binder with antimetastatic activity in a mouse model of melanoma | ||
Int J Biol Sci Septin4 Prevents PDGF-BB-induced HAVSMC Phenotypic Transformation, Proliferation and Migration by Promoting SIRT1-STAT3 Deacetylation and Dephosphorylation.
| ||
Biochem Biophys Res Commun Septin4 as a novel binding partner of PARP1 contributes to oxidative stress induced human umbilical vein endothelial cells injure.
| ||
Front Endocrinol (Lausanne) A novel mutation in CFAP47 causes male infertility due to multiple morphological abnormalities of the sperm flagella | ||
Biomed Pharmacother Atorvastatin rescues vascular endothelial injury in hypertension by WWP2-mediated ubiquitination and degradation of ATP5A | ||
Asian J Androl The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities |