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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEPT4 Recombinant antibody

SEPT4 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5O8

Cat no : 82284-1-RR

Synonyms

hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT4 Recombinant antibody (82284-1-RR)

    WB analysis using 82284-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEPT4 Recombinant antibody (82284-1-RR)

    IHC staining of mouse brain using 82284-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEPT4 Recombinant antibody (82284-1-RR)

    IHC staining of mouse brain using 82284-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82284-1-RR

    Affinity and Kinetic Characterization of 82284-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82284-1-RR against Human SEPT4 were performed. The affinity constant is 1.38 nM.

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82284-1-RR targets SEPT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEPT4 fusion protein Ag3185 相同性解析による交差性が予測される生物種
Full Name septin 4
Calculated molecular weight 459 aa, 53 kDa
Observed molecular weight38-55 kDa
GenBank accession numberBC018056
Gene symbol SEPT4
Gene ID (NCBI) 5414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).

Protocols

Product Specific Protocols
WB protocol for SEPT4 antibody 82284-1-RRDownload protocol
IHC protocol for SEPT4 antibody 82284-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols