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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT6 Monoclonal antibody (68173-1-Ig)

    WB analysis using 68173-1-Ig (same clone as 68173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68173-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEPT6 Monoclonal antibody (68173-1-Ig)

    IF Staining of HEK-293 using 68173-1-Ig (same clone as 68173-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody (68173-1-Ig, Clone: 1B1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68173-1-PBS in a different storage buffer formulation.

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SEPT6 Monoclonal antibody, PBS Only

SEPT6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68173-1-PBS
Clone No.1B1G1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68173-1-PBS targets SEPT6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Reactivity Human, mouse, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SEPT6 fusion protein Ag32736 相同性解析による交差性が予測される生物種
Full Name septin 6
Calculated molecular weight 434 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC036240
Gene Symbol SEPT6
Gene ID (NCBI) 23157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14141
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.