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  • Featured Product
  • KD/KO Validated

Septin 7 Polyclonal antibody

Septin 7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA, PLA

Conjugate

Unconjugated

Cat no : 13818-1-AP

Synonyms

septin7, SEPT7A, SEPT7, SEPT 7, CDC3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Septin 7 Polyclonal antibody (13818-1-AP)

    WB analysis using 13818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Septin 7 Polyclonal antibody (13818-1-AP)

    WB analysis of HepG2 using 13818-1-AP

    WB result of Septin 7 antibody (13818-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells.

  • Western Blot (WB) analysis of rat testis tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    WB analysis of rat testis using 13818-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    WB analysis of mouse testis using 13818-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Septin 7 Polyclonal antibody (13818-1-AP)

    WB analysis of Jurkat using 13818-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    IHC staining of human gliomas using 13818-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    IHC staining of human gliomas using 13818-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    IHC staining of human lung cancer using 13818-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    IHC staining of human lung cancer using 13818-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    IHC staining of human lung cancer using 13818-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Septin 7 Polyclonal antibody (13818-1-AP)

    IHC staining of human lung cancer using 13818-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE cells using Septin 7 Polyclonal antibody (13818-1-AP)

    IF Staining of hTERT-RPE cells using 13818-1-AP

    IF result of anti-Septin 7 (13818-1-AP, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 7. Blue: DNA. 1% PFA fixed.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Septin 7 Polyclonal antibody (13818-1-AP)

    IF Staining of HepG2 using 13818-1-AP

    Immunofluorescent analysis of HepG2 cells, using Septin 7 antibody 13818-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inA375 cells, HepG2 cells, Jurkat cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13818-1-AP targets Septin 7 in WB, IHC, IF/ICC, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 7 fusion protein Ag4907 相同性解析による交差性が予測される生物種
Full Name septin 7
Calculated molecular weight 51 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC025987
Gene symbol Septin 7
Gene ID (NCBI) 989
RRIDAB_2254298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

Protocols

Product Specific Protocols
WB protocol for Septin 7 antibody 13818-1-APDownload protocol
IHC protocol for Septin 7 antibody 13818-1-APDownload protocol
IF protocol for Septin 7 antibody 13818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Still and rotating myosin clusters determine cytokinetic ring constriction.

Authors - Viktoria Wollrab
View Article
humanWB

J Cell Biol

The septin cytoskeleton regulates natural killer cell lytic granule release.

Authors - Prasad V Phatarpekar
  • KD Validated
View Article
mouseWB,IF

Cell Rep

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Authors - Fernando Calvo
View Article
mouseIF,PLA

EMBO Rep

Cdc42-Borg4-Septin7 axis regulates HSC polarity and function

Authors - Ravinder Kandi
View Article
humanWB,IF

MBio

Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.

Authors - Quynh T Phan
  • KD Validated
View Article
bovineIF

Development

Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling.

Authors - Mengyun Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Amaury (Verified Customer) (03-29-2022)

I performed immunolabelling on K562. Used ice-cold methanol to permeabilize the cells and obtain very nice labelling

  • Applications: Immunofluorescence
  • Cell Tissue Type: K562
Septin 7 Antibody Immunofluorescence validation ( dilution) in K562 (Cat no:13818-1-AP)
FH

stephen (Verified Customer) (02-20-2019)

HeLa cells fixed with 4%PFA and permblised with 0.3% triton x100 for 5 mins. stained with septin 7ab 1:300. (green) and DAPI to mark DNA (blue). non dividing interphase cell (top). Dividing cell in anaphase (bottom)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HeLa cells
Septin 7 Antibody Immunofluorescence, validation (1:300 dilution) in HeLa cells (Cat no:13818-1-AP)