Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 13818-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13818-1-AP
WB result of Septin 7 antibody (13818-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells.
WB analysis of rat testis using 13818-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13818-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13818-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 13818-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13818-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13818-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13818-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13818-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 13818-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 13818-1-AP
Immunofluorescent analysis of HepG2 cells, using Septin 7 antibody 13818-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

Septin 7 Polyclonal antibody

Septin 7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13818-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA, PLA

Synonyms

septin7, SEPT7A, SEPT7, SEPT 7, CDC3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HepG2 cells, Jurkat cells, rat testis tissue, mouse testis tissue
Positive IHC detected in	human gliomas tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 21 publications below
IF	See 21 publications below
CoIP	See 1 publications below

Product Information

13818-1-AP targets Septin 7 in WB, IHC, IF/ICC, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Septin 7 fusion protein Ag4907 相同性解析による交差性が予測される生物種
Full Name	septin 7
Calculated molecular weight	51 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC025987
Gene Symbol	Septin 7
Gene ID (NCBI)	989
RRID	AB_2254298
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16181
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

Protocols

Product Specific Protocols
WB protocol for Septin 7 antibody 13818-1-AP	Download protocol
IHC protocol for Septin 7 antibody 13818-1-AP	Download protocol
IF protocol for Septin 7 antibody 13818-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nat Commun Still and rotating myosin clusters determine cytokinetic ring constriction. Authors - Viktoria Wollrab View Article
human	WB	J Cell Biol The septin cytoskeleton regulates natural killer cell lytic granule release. Authors - Prasad V Phatarpekar KD Validated View Article
mouse	WB,IF	Cell Rep Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts. Authors - Fernando Calvo View Article
mouse	IF,PLA	EMBO Rep Cdc42-Borg4-Septin7 axis regulates HSC polarity and function Authors - Ravinder Kandi View Article
human	WB,IF	MBio Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans. Authors - Quynh T Phan KD Validated View Article
bovine	IF	Development Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling. Authors - Mengyun Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13818-1-AP

WB analysis of HepG2 using 13818-1-AP

WB analysis of rat testis using 13818-1-AP

WB analysis of Jurkat using 13818-1-AP

WB analysis of mouse testis using 13818-1-AP

IHC staining of human gliomas using 13818-1-AP

IHC staining of human gliomas using 13818-1-AP

IHC staining of human lung cancer using 13818-1-AP

IHC staining of human lung cancer using 13818-1-AP

IHC staining of human lung cancer using 13818-1-AP

IHC staining of human lung cancer using 13818-1-AP

IF Staining of HepG2 using 13818-1-AP

Septin 7 Polyclonal antibody

Septin 7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Still and rotating myosin clusters determine cytokinetic ring constriction.

The septin cytoskeleton regulates natural killer cell lytic granule release.

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Cdc42-Borg4-Septin7 axis regulates HSC polarity and function

Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.

Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling.