Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10769-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 10769-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10769-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 10769-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10769-1-AP
IP result of anti-Septin 9 (IP:10769-1-AP, 4ug; Detection:10769-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human pancreas cancer using 10769-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10769-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10769-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10769-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10769-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of hTERT-RPE cells using 10769-1-AP
IF result of anti-Septin 9 (10769-1-AP, 1:200) with hTERT-RPE cell by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Green: SEPT9. Blue: DNA. Fixation in methanol.
IF Staining of HepG2 using 10769-1-AP
Immunofluorescent analysis of HepG2 cells, using Septin 9 antibody 10769-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of A431 using 10769-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10769-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 10769-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Septin 9 (10769-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Septin 9 Polyclonal antibody

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10769-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	C6 cells, HeLa cells, mouse spleen tissue, mouse brain tissue, mouse thymus tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human pancreas cancer tissue, human colon tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, hTERT-RPE cells, HepG2 cells, A431 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 23 publications below
IHC	See 2 publications below
IF	See 18 publications below

Product Information

10769-1-AP targets Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Septin 9 fusion protein Ag1218 相同性解析による交差性が予測される生物種
Full Name	septin 9
Calculated molecular weight	64 kDa
Observed molecular weight	65 kDa, 38-40 kDa
GenBank accession number	BC021192
Gene Symbol	SEPT9
Gene ID (NCBI)	10801
RRID	AB_2185012
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UHD8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Protocols

Product Specific Protocols
WB protocol for Septin 9 antibody 10769-1-AP	Download protocol
IHC protocol for Septin 9 antibody 10769-1-AP	Download protocol
IF protocol for Septin 9 antibody 10769-1-AP	Download protocol
IP protocol for Septin 9 antibody 10769-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IF	Dev Cell Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin. Authors - Eva P Karasmanis KD Validated View Article
canine	IF	Curr Biol A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. Authors - Eva P Karasmanis KD Validated View Article
human	WB,IF	Curr Biol Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules. Authors - Joëlle Salameh View Article
human	WB, IF	J Cell Biol A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport. Authors - Ilona A Kesisova View Article
human	IF	J Cell Biol Septins suppress the release of vaccinia virus from infected cells. Authors - Julia Pfanzelter View Article
mouse	WB	PLoS Genet Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis. Authors - Manoj B Menon View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10769-1-AP

WB analysis using 10769-1-AP

WB analysis of HeLa using 10769-1-AP

WB analysis of mouse spleen using 10769-1-AP

IP experiment of mouse brain using 10769-1-AP

IHC staining of human pancreas cancer using 10769-1-AP

IHC staining of human pancreas cancer using 10769-1-AP

IHC staining of human colon using 10769-1-AP

IHC staining of human testis using 10769-1-AP

IHC staining of human testis using 10769-1-AP

IF Staining of hTERT-RPE cells using 10769-1-AP

IF Staining of HepG2 using 10769-1-AP

IF Staining of A431 using 10769-1-AP

IF Staining of HeLa using 10769-1-AP

FC experiment of HepG2 using 10769-1-AP

Septin 9 Polyclonal antibody

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

Septins suppress the release of vaccinia virus from infected cells.

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.