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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERBP1 Monoclonal antibody (67792-1-Ig)

    WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDCK cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    WB analysis of MDCK using 67792-1-Ig (same clone as 67792-1-PBS)

    MDCK cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    WB analysis of HSC-T6 using 67792-1-Ig (same clone as 67792-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SERBP1 Monoclonal antibody (67792-1-Ig)

    IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERBP1 Monoclonal antibody (67792-1-Ig)

    IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERBP1 Monoclonal antibody (67792-1-Ig)

    IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SERBP1 Monoclonal antibody (67792-1-Ig)

    IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

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SERBP1 Monoclonal antibody, PBS Only

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 67792-1-PBS
Clone No.1D4H1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, Canine

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67792-1-PBS targets SERBP1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples.

Tested Reactivity Human, Mouse, Rat, pig, Canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERBP1 fusion protein Ag30202 相同性解析による交差性が予測される生物種
Full Name SERPINE1 mRNA binding protein 1
Calculated molecular weight 45 kDa
Observed molecular weight50 kDa
GenBank accession numberBC008045
Gene Symbol SERBP1
Gene ID (NCBI) 26135
RRIDAB_2918556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NC51
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).