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SERCA2,ATP2A2 Polyclonal antibody
SERCA2,ATP2A2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IF-P, IF-Fro, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 27311-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, HEK-293 cells, mouse heart unboiled, 37°C incubated HEK-293 cells |
| Positive IHC detected in | mouse skeletal muscle tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
27311-1-AP targets SERCA2,ATP2A2 in WB, IHC, IF/ICC, IF-P, IF-Fro, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SERCA2,ATP2A2 fusion protein Ag26177 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| Calculated molecular weight | 1042 aa, 115 kDa |
| Observed molecular weight | 110-115 kDa |
| GenBank accession number | BC035588 |
| Gene symbol | SERCA2 |
| Gene ID (NCBI) | 488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SERCA2,ATP2A2 antibody 27311-1-AP | Download protocol |
| IHC protocol for SERCA2,ATP2A2 antibody 27311-1-AP | Download protocol |
| IF protocol for SERCA2,ATP2A2 antibody 27311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JCI Insight Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy. | ||
Nat Commun ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome | ||
Biochem Biophys Res Commun Mitigation of ROS-triggered endoplasmic reticulum stress by upregulating Nrf2 retards diabetic nephropathy | ||
FASEB J Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis |
