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Species Reactivity

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SERCA2,ATP2A2 Monoclonal antibody, PBS Only

SERCA2,ATP2A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F11A11

Cat no : 67248-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    WB analysis of HEK-293 using 67248-1-Ig (same clone as 67248-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    WB analysis of HepG2 using 67248-1-Ig (same clone as 67248-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    WB analysis of MCF-7 using 67248-1-Ig (same clone as 67248-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    WB analysis of HSC-T6 using 67248-1-Ig (same clone as 67248-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    WB analysis of NIH/3T3 using 67248-1-Ig (same clone as 67248-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SERCA2,ATP2A2 Monoclonal antibody (67248-1-Ig)

    IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67248-1-PBS targets SERCA2,ATP2A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERCA2,ATP2A2 fusion protein Ag5052 相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Calculated molecular weight 1042 aa, 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC035588
Gene symbol SERCA2
Gene ID (NCBI) 488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.