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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SERCA2,ATP2A2 Monoclonal antibody

SERCA2,ATP2A2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F11A11

Cat no : CL488-67248

Synonyms

ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SERCA2,ATP2A2 Monocl (CL488-67248)

    FC experiment of HeLa using CL488-67248

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SERCA2,ATP2A2 (CL488-67248, Clone:1F11A11) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67248 targets SERCA2,ATP2A2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERCA2,ATP2A2 fusion protein Ag5052 相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Calculated molecular weight 1042 aa, 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC035588
Gene symbol SERCA2
Gene ID (NCBI) 488
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SERCA2,ATP2A2 antibody CL488-67248Download protocol
Standard Protocols
Click here to view our Standard Protocols