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  • Featured Product
  • KD/KO Validated

SERF2 Polyclonal antibody

SERF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11691-1-AP

Synonyms

small EDRK rich factor 2, Protein 4F5-related, Protein 4F5 related, h4F5rel, Gastric cancer-related protein VRG107

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SERF2 Polyclonal antibody (11691-1-AP)

    WB analysis of HepG2 using 11691-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11691-1-AP (SERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SERF2 Polyclonal antibody (11691-1-AP)

    IP experiment of HepG2 using 11691-1-AP

    IP result of anti-SERF2 (IP:11691-1-AP, 4ug; Detection:11691-1-AP 1:500) with HepG2 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SERF2 Polyclonal antibody (11691-1-AP)

    IHC staining of human skin cancer using 11691-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 11691-1-AP (SERF2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SERF2 Polyclonal antibody (11691-1-AP)

    IF Staining of A549 using 11691-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SERF2 antibody (11691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SERF2 Polyclonal antibody (11691-1-AP)

    IF Staining of MCF-7 using 11691-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SERF2 antibody (11691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11691-1-AP targets SERF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERF2 fusion protein Ag2305 相同性解析による交差性が予測される生物種
Full Name small EDRK-rich factor 2
Calculated molecular weight 59 aa, 7 kDa
GenBank accession numberBC022326
Gene symbol SERF2
Gene ID (NCBI) 10169
RRIDAB_10596937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERF2 Belongs to the SERF family. The antibody is specific to SERF2.

Protocols

Product Specific Protocols
WB protocol for SERF2 antibody 11691-1-APDownload protocol
IHC protocol for SERF2 antibody 11691-1-APDownload protocol
IF protocol for SERF2 antibody 11691-1-APDownload protocol
IP protocol for SERF2 antibody 11691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mamm Genome

A novel knockout mouse for the small EDRK-rich factor 2 (Serf2) showing developmental and other deficits.

Authors - Karen Cleverley
  • KD Validated
View Article
mouseWB

Life Sci Alliance

Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brain

Authors - Esther Stroo
View Article