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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SERP1 Polyclonal antibody (17807-1-AP)

    WB analysis of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SERP1 Polyclonal antibody (17807-1-AP)

    WB analysis of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using SERP1 Polyclonal antibody (17807-1-AP)

    WB analysis of mouse pancreas using 17807-1-AP (same clone as 17807-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SERP1 Polyclonal antibody (17807-1-AP)

    IP experiment of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

    IP result of anti-SERP1 (IP:17807-1-AP, 4ug; Detection:17807-1-AP 1:500) with mouse brain tissue lysate 3160 ug. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using SERP1 Polyclonal antibody (17807-1-AP)

    IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using SERP1 Polyclonal antibody (17807-1-AP)

    IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SERP1 Polyclonal antibody (17807-1-AP)

    IF Staining of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERP1 antibody (17807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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SERP1 Polyclonal antibody, PBS Only

SERP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17807-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Stress-associated endoplasmic reticulum protein 1, Ribosome-attached membrane protein 4, RAMP4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17807-1-PBS targets SERP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERP1 fusion protein Ag12087 相同性解析による交差性が予測される生物種
Full Name stress-associated endoplasmic reticulum protein 1
Calculated molecular weight 66 aa, 7 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC108314
Gene Symbol SERP1
Gene ID (NCBI) 27230
RRIDAB_10597394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6X1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome- associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress [PMID:16705175]. SERP1 interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. It controls glycosylation of major histocompatibility complex class II-associated invariant chains by a translocational pausing mechanism, and its overexpression stabilizes newly synthesized membrane proteins under ER stress by associating with the Sec61 complex [PMID:10601334]. It is suggested SERP1 is involved in the biosynthesis/processing of secretory proteins