SERPINA10 Polyclonal antibody

SERPINA10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11851-1-AP

Synonyms

ZPI, UNQ707/PRO1358, Serpin A10, PZI, PZ-dependent protease inhibitor


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Tested Applications

Positive WB detected inhuman liver tissue, HepG2 cells, L02 cells, mouse liver tissue, RAW264.7
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11851-1-AP targets SERPINA10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINA10 fusion protein Ag2420 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Calculated molecular weight 444 aa, 51 kDa
Observed molecular weight 72 kDa, 68 kDa, 50-55 kDa
GenBank accession numberBC022261
Gene symbol SERPINA10
Gene ID (NCBI) 51156
RRIDAB_2254343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SERPINA10 antibody 11851-1-APDownload protocol
IHC protocol for SERPINA10 antibody 11851-1-APDownload protocol
IF protocol for SERPINA10 antibody 11851-1-APDownload protocol
IP protocol for SERPINA10 antibody 11851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols