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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPINA10 Monoclonal antibody (60113-1-Ig)

    WB analysis using 60113-1-Ig (same clone as 60113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60113-1-Ig (SERPINA10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SERPINA10 Monoclonal antibody (60113-1-Ig)

    IHC staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SERPINA10 Monoclonal antibody (60113-1-Ig)

    IHC staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using SERPINA10 Monoclonal antibody (60113-1-Ig)

    IF Staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using SERPINA10 Monoclonal antibody (60113-1-Ig)

    IF Staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPINA10 Monoclonal antibody (60113-1-Ig)

    IF Staining of HepG2 using 60113-1-Ig (same clone as 60113-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

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SERPINA10 Monoclonal antibody, PBS Only

SERPINA10 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60113-1-PBS
Clone No.5B3C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

5B3C9, Protein Z-dependent protease inhibitor, PZ-dependent protease inhibitor, PZI, Serpin A10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60113-1-PBS targets SERPINA10 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SERPINA10 fusion protein Ag2420 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Calculated molecular weight 444 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC022261
Gene Symbol SERPINA10
Gene ID (NCBI) 51156
RRIDAB_2301526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UK55
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SERPINA10 belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis.