Protein C inhibitor Polyclonal antibody

Name: Protein C inhibitor Polyclonal antibody
Brand: Proteintech
SKU: 10673-1-AP

Protein C inhibitor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10673-1-AP

Synonyms

Serpin A5, PROCI, Plasminogen activator inhibitor 3, Plasma serine protease inhibitor, PLANH3

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HepG2 using 10673-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10673-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10673-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10673-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10673-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10673-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10673-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 10673-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 10673-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 10673-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10673-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 10673-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 10673-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10673-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, PC-3 cells
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:600
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 5 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

Product Information

10673-1-AP targets Protein C inhibitor in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Protein C inhibitor fusion protein Ag1078 相同性解析による交差性が予測される生物種
Full Name	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
Calculated molecular weight	46 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC008915
Gene symbol	SERPINA5
Gene ID (NCBI)	5104
RRID	AB_2185741
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

Protocols

Product Specific Protocols
WB protocol for Protein C inhibitor antibody 10673-1-AP	Download protocol
IHC protocol for Protein C inhibitor antibody 10673-1-AP	Download protocol
IF protocol for Protein C inhibitor antibody 10673-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Mod Pathol Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors. Authors - Bijsmans Ingrid T G W IT View Article
human	IHC	J Neuropathol Exp Neurol Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes. Authors - Rodriguez Fausto J FJ View Article
rat	IHC	Proteomics Clin Appl Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis. Authors - Tengjiao Zhu View Article
human	WB,CoIP	J Cell Mol Med SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer Authors - Meiyang Fan View Article
rat	ELISA,IHC	Biomolecules Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression Authors - Shanshui Zeng View Article
human,rat	IHC	J Proteome Res Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis Authors - Qizhao Tan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Protein C inhibitor Polyclonal antibody

Protein C inhibitor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 10673-1-AP

WB analysis of HepG2 using 10673-1-AP

WB analysis of L02 using 10673-1-AP

WB analysis of Jurkat using 10673-1-AP

WB analysis of A549 using 10673-1-AP

WB analysis of HeLa using 10673-1-AP

WB analysis of PC-3 using 10673-1-AP

IHC staining of human ovary tumor using 10673-1-AP

IHC staining of human ovary tumor using 10673-1-AP

IHC staining of human testis using 10673-1-AP

IHC staining of human testis using 10673-1-AP

IHC staining of human breast cancer using 10673-1-AP

IF Staining of HepG2 using 10673-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors.

Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.

Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis.

SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer

Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression

Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis